Showing: 1 - 20 of 1982 results
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rnALDH
Plasmid#67920PurposeExpresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coliDepositorInsertRattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
UseTags6x-HisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-P2A-strepII-PPPCB
Plasmid#67923PurposeExpresses mCherry and strepII-tag-PP2aBeta in mammalian cellsDepositorInsertmCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 VAMP2 R125TAG
Plasmid#69876Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2-GFPDepositorInsertVAMP2-GFP (Vamp2 Rat)
UseSynthetic BiologyTagsGFPExpressionMammalianMutationAmber stop codon in the linker between VAMP2 and …PromoterAvailabilityAcademic Institutions and Nonprofits only -
pN1 VAMP2 R125TAG
Plasmid#69877Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2DepositorInsertVAMP2 (Vamp2 Rat)
UseSynthetic BiologyTagsExpressionMammalianMutationAmber and Ochre stop codons in the former linker …PromoterAvailabilityAcademic Institutions and Nonprofits only -
Cx43-sfGFP
Plasmid#70053PurposeExpresses rat Cx43 (Gja1 CDS) tagged with non-monomerized sfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsnon-monomerized superfolder GFP (V207)ExpressionMutationfused in frame to non-monomerized superfolder GFPPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDC184
Plasmid#70055PurposeCo-expresses Syt1-SNARE37aa-linker complex with p045DepositorInsertsHis tagged Synaptobrevin 28-89
Syntaxin 191-256
UseTags10xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
p045
Plasmid#70057PurposeCo-expresses Syt1-SNARE37aa-linker complex with pDC184DepositorInsertsC2AB-linker-SNAP-25_C
SNAP-25_N
UseTagsNo tagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-C1-rGAT
Plasmid#70107Purposemammalian expression of rat GAT with N terminal YFP fusionDepositorInsertGAT (Slc6a1 Rat)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pECFP-C1-rGAT
Plasmid#70108Purposemammalian expression of rat GAT with N terminal CFP fusionDepositorInsertGAT (Slc6a1 Rat)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-pHoenix-WPRE
Plasmid#70111Purposelentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesiclesDepositorInsertpHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
UseLentiviralTagsArch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…ExpressionMutationPromoterSynapsinAvailabilityAcademic Institutions and Nonprofits only -
pCMV-lyso-pHoenix
Plasmid#70112PurposeExpression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomesDepositorInsertlyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse, Rat)
UseTagsArch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-PLCdelta-PH
Plasmid#71375PurposeFor analysis of PH domain localization in yeastDepositorInsertPLCdelta-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domainPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenSyn2-EGFP-OMP25C
Plasmid#71427PurposeLentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.DepositorInsertOMP25 (172-‐206) (Synj2bp Rat)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 172-206 onlyPromotersynapsinAvailabilityAcademic Institutions and Nonprofits only -
CD200-Cd4d3+4-COMP-blac-3xFLAG-6his
Plasmid#71471PurposeA positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kitDepositorInsertCD200 (Cd200 Rat)
UseTags6xHis tag, Cd4d3+4, beta-lactamase, and triple FL…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Cx43-msfGFP
Plasmid#69007PurposeExpresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertGja1 (Gja1 Rat)
UseTagsmonomerized superfolder GFPExpressionMammalianMutationPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
Cx26-msfGFP
Plasmid#69016PurposeExpresses rat Cx26 (Gjb2 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.DepositorInsertCx26 (Gjb2 Rat)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx43
Plasmid#69020PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsEBFP2ExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorUseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Cx43K258stop-msfGFP
Plasmid#69023PurposeExpresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.DepositorInsertCx43 (Gja1 Rat)
UseTagsV206K monomerized super folder GFPExpressionMammalianMutationdelete codons 258-381 and fuse in frame to monome…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 1 - 20 of 1982 results