Making LB Agar Plates |
Create plates to culture bacteria in the lab |
Pouring LB Agar Plates Protocol |
Streaking Bacteria on Plates |
Isolate single bacterial colonies on an agar plate |
Streaking Bacteria on Plates Protocol |
Diagnostic Restriction Digest |
Verify a plasmid using restriction enzymes |
Diagnostic Restriction Digest Protocol |
Storing and Handling Addgene Plasmids |
What to do after you receive your plasmids from Addgene |
Instructions for Storing and Handling Plasmids |
Genomic Deletions with CRISPR |
Generate genomic deletions in mammalian cells lines using CRISPR/Cas9 |
Genomic Deletions with CRISPR Protocol |
Over-Agar Antibiotic Plating |
Create plates to culture bacteria in the lab |
Over-Agar Antibiotic Plating Protocol |
AAV Purification |
Purify adeno-associated virus from a preparation produced in mammalian cells |
AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol |
Mulitchannel Pipetting Technique |
Learn how to pipette multiple samples at once using a multichannel pipette and avoid inconsistent pipetting with our simple tips |
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Bacterial Transformations |
We walk you through a standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and increase efficiency |
Bacterial Transformation Protocol |
Inoculating a Liquid Bacterial Culture |
How to prepare and grow bacteria in a liquid medium |
Inoculating a Liquid Bacterial Culture Protocol |
Getting Started with Tissue Culture |
Tips and tricks for working with tissue culture in the lab. |
Blog post: 10 Basic tips for mammalian cell culture |
Aseptic Technique |
Aseptic technique is a critical skill for success in numerous fields such as microbiology, molecular biology or cell biology. Learn some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean workspace, and maintaining sterility while working. |
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AAV Titration by qPCR |
Use qPCR to measure the physical titer of your adeno-associated virus preparation. |
AAV Titration by qPCR Using SYBR Green Technology Protocol |
Agarose Gel Electrophoresis |
Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization and purification. |
Agarose Gel Electrophoresis Protocol |
Purifying DNA from an Agarose Gel |
Gel purification allows you to isolate and purify DNA fragments based on size. Following gel electrophoresis, you can cut DNA bands out of the agarose gel and purify the DNA samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. |
Purifying DNA from an Agarose Gel Protocol |