Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.
Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W
Genetics. 2015 Apr 8. pii: genetics.115.176917. PubMed Article
Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W
Genetics. 2015 Apr 8. pii: genetics.115.176917. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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64237 | pME-Cas9 | expresses codon optimized Cas9 in zebrafish |
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64239 | pGGDestTol2LC-1sgRNA | destination vector for one U6x:sgRNA cassette |
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64240 | pGGDestTol2LC-2sgRNA | destination vector for two U6x:sgRNA cassettes |
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64241 | pGGDestTol2LC-3sgRNA | destination vector for three U6x:sgRNA cassettes |
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64242 | pGGDestTol2LC-4sgRNA | destination vector for four U6x:sgRNA cassettes |
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64243 | pGGDestTol2LC-5sgRNA | destination vector for five U6x:sgRNA cassettes |
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64245 | pU6a:sgRNA#1 | expresses sgRNA under U6a promoter |
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64246 | pU6a:sgRNA#2 | expresses sgRNA under U6a promoter |
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64247 | pU6b:sgRNA#3 | expresses sgRNA under U6b promoter |
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64248 | pU6c:sgRNA#4 | expresses sgRNA under U6c promoter |
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64249 | pU6d:sgRNA#5 | expresses sgRNA under U6d promoter |
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64250 | pU6a:sgRNA(tyr) | expresses sgRNA(tyr) under U6a promoter |
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