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Purpose(Empty Backbone) pRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47916 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRetro-X-Tight
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Backbone manufacturerclontech
- Backbone size (bp) 6536
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Modifications to backbonePuromycin resistance gene replaced with Nourseothricin resistance gene
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Vector typeMammalian Expression, Retroviral
- Promoter Tet
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Selectable markersNourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN_5_primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe original NAT ORF was obtained from Prof Laura Knoll, University of Wisconsin-Madison. Sequence of the ORF was partially adapted to human codon usage.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRXTN was a gift from James Iglehart (Addgene plasmid # 47916 ; http://n2t.net/addgene:47916 ; RRID:Addgene_47916) -
For your References section:
Nourseothricin N-acetyl transferase: a positive selection marker for Mammalian cells. Kochupurakkal BS, Iglehart JD. PLoS One. 2013 Jul 4;8(7):e68509. doi: 10.1371/journal.pone.0068509. Print 2013. 10.1371/journal.pone.0068509 PubMed 23861913