pHEF-VSVG
(Plasmid
#22501)
-
Depositing Lab
-
Publication
-
Sequence Information
-
Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
-
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 22501 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
Backbone
-
Vector backboneN/A
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Growth instructionsTransform into DH5alpha and grow in Luria Broth containing 100µg/ml ampicillin.
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameVesicular Stomatitis Virus Glycoprotein
-
SpeciesRhabdoviridae
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
-
Addgene Notes
-
A portion of this plasmid was derived from a plasmid made byOriginal plasmid sent by Jason Coleman and Matthew Huentelman.
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid forms part of a plasmid set for lentivirus generation in the Kasparov lab, University of Bristol, UK.
Addgene was unable to obtain quality control sequence for this plasmid. The depositing lab suggested the following diagnostic digest: PstI digest, expect 0.55, 1.3, and 4.0 kb fragments. Our results for the digest can be seen under "Addgene Notes" on this page.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHEF-VSVG was a gift from Sergey Kasparov (Addgene plasmid # 22501 ; http://n2t.net/addgene:22501 ; RRID:Addgene_22501) -
For your References section:
Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Coleman JE, Huentelman MJ, Kasparov S, Metcalfe BL, Paton JF, Katovich MJ, Semple-Rowland SL, Raizada MK. Physiol Genomics. 2003 Feb 6. 12(3):221-8. 10.1152/physiolgenomics.00135.2002 PubMed 12488511
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/13/28/c29ceae4-a9c1-11e1-b406-003048dd6500.png.940x940_q85_autocrop.png)